Bone (cross section)

		Bone (cross section)

Workbook tasks

Slide 12: Bone

H/E

This is a cross section through decalcified bone. There are two ways to study bone histology. Dry bone is cut and polished before mounting on a slide. Bone decalcification is the removal of the mineral component using an acid, leaving the bone soft and easy to cut. In dry ground bone, the organic material is removed. In contrast, in decalcified slides the relationship between the living components of bone is retained.

Identify:

Macroscopic

Compact decalcified stained bone

Microscopic

Haversian and Volkmann's canals

Lacunae
Bone matrix
Periosteum
Fat cells of the bone marrow

Draw and annotate:

A section of bone in which Haversian systems and periosteum are indicated.

Reflection:

What is present in the Haversian canals? What is their function?
How are the lacunae arranged and what is found in them? What is their function?

Slides

Cross section through decalcified bone

Osteoblasts

Osteoklasts

Medium magnification of osteoklasts in immature bone

Very low magnification

Very low magnification of decalcified bone

Medium magnification

High magnification

High magnification of bone-remodeling unit in immature bone (PAS stain)
High magnification of immature bone

High magnification of resorption cavity
High magnification of osteoclast progenitor cells
High magnification of a bone remodeling unit
High magnification of bone-remodeling unit in immature bone
High magnification of osteoclast progenitor cells

Very high magnification

Very high magnification of an osteoclast