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Slide 33: Coronal section through the tongue
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H/E
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This slide is a coronal section through the tip of the tongue. The skeletal muscle is sectioned longitudinally, obliquely and crosswise.
The slide is used to study skeletal muscle and papilla circumvalata.
See also slide 18, a longitudinal and cross section of skeletal muscle.
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To view the striations of skeletal muscle in an H/E specimen, the condenser of the microscope must be turned down to obtain as much contrast as possible. (If you used a microscope, remember to turn the condenser back to its original position after you have finished).
Identify microscopically:
- Longitudinal section
- Skeletal muscle fibers
- Nuclei of the skeletal muscle fibers and connective tissue
- Cross striations
- Connective tissue
- Cross section
- Skeletal muscle fibers arranged in bundles
- Size, shape and diameter of the muscle bundles
- Nuclei of the skeletal muscle fibers
- Myofibrils
- Connective tissue
- On and below the surface
- Papilla circumvalata
- Glands of Ebner
- Taste buds
Draw and annotate:
A longitudinal and cross section of skeletal muscle.
A circumvallate papilla and associated gland.
Reflection:
- What is characteristic of skeletal muscle?
- What is characteristic of the nuclei of skeletal muscle and how do they differ from those of smooth muscle?
- How does skeletal muscle differ in longitudinal and cross section from smooth muscle?
- What are skeletal muscle fibre bundles called?
- Can all the subdivisions of muscle connective tissue be seen on this specimen? What is the connective tissue is called?
- What is the shape of the papilla circumvallata?
- Where do the glands of Ebner open?
- What type of gland is the gland of Ebner?
- Where are the taste buds found?
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