Slide 94: Thin skin
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H/E
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This slide is a section through thin skin from the thigh region.
Thin skin is used to demonstrate sectioned loose connective tissue and dense irregular connective tissue.
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Identify:
Macroscopic
The different staining layers of thin skin.
A fine dark area, the epidermis A thick lighter eosinophilic area, the dermis A very thick light area with a fine eosinophilic line running through, the hypodermis.
Microscopic
The dermis is that part of the skin just beneath the epidermis (epithelium). It is divided into two parts, namely the papillary and reticular layers. The papillary layer is situated directly beneath the epidermis and consists of loose connective tissue. The reticular part is composed of dense irregular connective tissue.
The layers of the skin and hypodermis Sweat glands Hair Sebaceous glands Dense irregular connective tissue [is present in the reticular part i.e. the deeper part of the dermis] Fibroblast nuclei. Collagen fibers sectioned in different planes Nuclei of the connective tissue cells
Draw and annotate:
The layers of thin skin, including the associated structures: Show the relationships between the different layers.
Dense irregular connective tissue. Indicate the collagen fibers sectioned in different planes.
Collagen fibers sectioned in different directions also indicate the different connective tissue nuclei.
Reflection:
- What is characteristic of skin from the thigh region?
- How do the sectioned collagen fibers differ from those of the mesenterium as spread out [compare slide 6]?
- What is the relative composition of this tissue, with regard to % fibers, cells and amorphous ground substance?
- How does the fibers of dense irregular connective tissue differ from loose connective tissue in a tissue section?
- Are there more or less cells than in loose connective tissue?
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