07 Blood

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07 BloodWhite blood cellsLeukocytesGranularNongranular5 * 109 - 9 * 109Differential white cell countPlateletsRed blood cells
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07 Blood
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White blood cells
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Leukocytes
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Granular
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Neutrophils
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50% - 70%
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nucleus 2 - 5 lobes
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10 hours + 1-2 days
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Eosinophils
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eosinophilic
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1% - 4%
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2 lobes
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acidophilic granules
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Basophils
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basophilic
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<1%
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blue-staining granules
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Nongranular
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Lymphocytes
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lymph and blood
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20% - 40%
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Monocytes
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macrophage precursors
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2% - 8%
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5 * 109 - 9 * 109

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Differential white cell count
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Shape
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Complete cell must be visible
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Shape should be normal
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Round
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Size
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Key = White blood cells
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=scale
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Large white blood cells x3
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neutrophils
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eosinophils
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basophils
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Small white blood cells x2
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lymphocytes
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monocytes
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Nucleus
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Clear lobes
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neutrophils
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eosinophils
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Two lobes
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eosinophils
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basophils
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Many lobes
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neutrophils
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No or undefined lobes
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basophils
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lymphocytes
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monocytes
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Cytoplasm
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Relative to nucleus
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More than
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neutrophils
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eosinophils
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Equal
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monocytes
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Less
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basophils
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lymphocytes
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Granules
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Key for colour is red blood cells and nucleus
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Same staining method
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BUT
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Result can vary
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red blood cell
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granules of eosinophil
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nucleus
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granules of basophil
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Artifacts
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dust
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bubbles
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damaged cells
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are not counted
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remove artifacts
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remove red blood cells
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count remaining
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Platelets
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150 * 109 - 400 * 109 / liter

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2-3 µm
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10 days
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hemostasis
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Red blood cells
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5 * 1012 / liter

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7 µm

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4 month
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