01 Microscopy

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  1. Oldest design of microscope from around 1600 constructed by johannes and zaccharias janssen. Generally consist of light source, condenser, objective lens and an ocular or eyepiece. (18)
  2. A lens designed to focus light from the illumination source onto the sample. (9)
  3. An objective designed to be used with a thin layer of immersion oil bridging the gap between the specimen and the bottom of the objective. (23)
  4. First part of the imaging system, closest to the object. The objective forms the primary image, which is then further magnified by the eyepiece. (9)
  5. A thin opaque structure with an opening at its center, used to manage the intensity of the illumination. (9)
  6. A steel knife mounted in a microtome is used to cut thin tissue sections which are mounted on a glass microscope slide (10)
  7. A mechanical device part of or connected to the stage for holding and moving the slide in the x or y direction in a precise distance by turning a knob. (16)
  8. Used to stain blood cells, in various colours, purple, pink, bright red, orange, deep purple, violet. (13)
  9. A means of adjusting the focus to finer limits, used after the image has been roughly focused using the coarse focus. (15)
  10. Reproductive cells (spermatozoa in men, oocytes in women) (10)
  11. The lining of pleural and pericardial spaces (11)
  12. A platform below the objective which supports the specimen being viewed. (5)
  13. A light or a mirror (12)
  14. Used to stain reticular and nerve fibers brown to black. (12)
  15. Cylinder containing two or more lenses to bring the image into focus for the eye (8)
  16. The rough focus knob on the microscope, changing the distance between the objective and the slide, to bring the specimen into rough focus. Use before fine focus and then continue with fine focus when changing objectives. In different models, the moving part can be the stage, the limb or the body tube. (12)
  17. Requiring immersion oil between objective front lens and specimen surface. Oil immersion is used to eliminate refraction. (13)
  18. Produces images of a sample by scanning the surface with a focused beam of electrons. (27)
  19. Study of living cells, their dna and rna and the proteins they express. (12)
  20. Most commonly used fixative for light microscopy. (8)
  21. Adjustment knobs move the stage up and down with separate adjustment for coarse and fine focusing (11)
  22. Studies entire organisms. (10)
  23. Collect light from the sample. (14)
  24. Microscope with a lens close to the object being viewed to collect light (called the objective lens) which focuses a real image of the object inside the microscope. That image is then magnified by a second lens or group of lenses (called the eyepiece) that gives the viewer an enlarged inverted virtual image of the object. (19)
  25. Low-powered microscope which provides a stereoscopic view of the sample, commonly used for dissection. (17)
  26. Remove water from tissues and replace with a medium that solidifies to allow thin sections to be cut. (17)
  27. Type of microscope which uses visible light and a system of lenses to magnify images of small samples. (16)
  28. Cells filling the spaces between the organs, including fat, muscle, bone, cartilage, and tendon cells (10)
  29. Using microscopes to view objects and areas of objects that cannot be seen with the naked eye. (10)


  1. Tissue samples placed into molds along with liquid embedding material which is then hardened. (9)
  2. Study of organs visible by the naked eye (7)
  3. Support, connect or separate tissues and organs. (17)
  4. Holds a set of objective lenses. It allows the user to switch between objective lenses. (16)
  5. Attaching the samples to a glass microscope slide for observation and analysis (8)
  6. Product of the powers of the ocular (eyepiece) and the objective lens. (13)
  7. Nuclear stain stains nuclei blue-violet or brown (12)
  8. A tool used to cut extremely thin slices of material, known as sections (9)
  9. An instrument used to see objects that are too small to be seen by the naked eye (10)
  10. A microscope that uses an ultraviolet light source to illuminate specimens that will fluoresce. Usually a fluorescent dye or antibody has been added to the specimen that is being viewed. (23)
  11. Lines cavities and surfaces in the body (17)
  12. A microscope that uses electrons, instead of visible light, to produce an image. This allows improved visualisation of fine structures, for example viruses, cellular components and small molecules. (19)
  13. Used to preserve tissue from degradation, and maintain the structure of the cell and sub-cellular components (8)
  14. A mounting point for various microscope controls. (5)
  15. A beam of electrons is transmitted through a specimen to form an image. (31)
  16. Lining of glands, bowel, skin, and some organs like the liver, lung, and kidney (10)
  17. Most commonly used fixative for electron microscopy. (14)
  18. Conducting cells of the nervous system (7)
  19. Biological tissue has little inherent contrast. Staining is employed to give both contrast to the tissue as well as highlighting particular features of interest. (8)
  20. The growth of tissues or cells separate from the organism. (14)
  21. Lining of blood and lymphatic vessels (11)
  22. Microscopic study of loose cells or clusters obtained from bodily secretions, aspirations, scrapes, swipes, or washings (8)
  23. Structures or features in tissue that interfere with normal histological examination (9)
  24. Cells with the ability to develop into different cell types (10)
  25. A means of moving the specimen closer or further away from the objective lens to obtain a sharp image. Rack-and-pinion is a popular type of focus mechanism. (5)
  26. A lens or set of lenses to enlarge an object through angular magnification alone, giving the viewer an erect enlarged virtual image, such as a magnifying glass. (17)
  27. Main tissue component of the central and peripheral nervous system (14)
  28. Eyepiece (7)
  29. A thin flat piece of transparent material placed over objects for viewing with a microscope (10)
  30. A thin flat piece of glass used to hold objects for examination under a microscope (16)
  31. Red and white blood cells, including those found in lymph nodes and spleen (11)
  32. Tissues with the ability to contract. (13)
  33. Study of the microscopic anatomy (microanatomy) of cells and tissues of plants and animals. (9)
  34. Manage the quality of the illumination. (7)
  35. Imparts a pink or red colour to cytoplasmic material, cell membranes, and some extracellular structures and a red colour to red blood cells. (5)

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